Coding

Part:BBa_K2029004:Design

Designed by: Niklas Schmacke, Max Schelski   Group: iGEM16_UBonn_HBRS   (2016-10-14)


CelA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part codes for cellulase celA from Clostridium thermocellum. It has been expressed and secreted in Bacillus subtilis strain WB800, behind the P43 promoter and the nprb secretion signal using plasmid pP43JM2 (ref 1)


Source

Clostridium thermocellum

References

1 DOI 10.1007/s12010-011-9456-z Cloning of Thermostable Cellulase Genes of Clostridium thermocellum and Their Secretive Expression in Bacillus subtilis